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DRG International
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Bachem
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DRG Diagnostics GmbH
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Merck KGaA
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Effect of Inflammatory Mediators Lipopolysaccharide and Lipoteichoic Acid on Iron Metabolism of Differentiated SH-SY5Y Cells Alters in the Presence of BV-2 Microglia
doi: 10.3390/ijms20010017
Figure Lengend Snippet: Effects of LPS and LTA treatments on hepcidin secretion and on iron content of SH-SY5Y cells. Hepcidin content of cell culture supernatants was determined with Hepcidin 25 bioactive ELISA Kit. The hepcidin contents of the control media did not change during the experiments therefore only one control was represented in Figure A. Intracellular iron contents of SH-SY5Y cells were determined using a colorimetric ferrozine-based assay. Iron contents of the untreated control cells were measured the same way and at the same time points of the experiments (6 h, 24 h, and 48 h). ( A ) Hepcidin concentrations in the cell culture media of the LPS and LTA treated SH-SY5Y monocultures. ( B ) Total iron contents of LPS and LTA treated SH-SY5Y cells. The columns represent mean values and error bars represent standard errors of the mean (SEM) of four independent determinations. ND stands for not detected. Asterisk marks p < 0.01 between LPS and LTA treatments. Cross marks p < 0.01 compared to the untreated controls.
Article Snippet: The mature hepcidin content of each sample was determined with
Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Ferrozine Assay
Journal: International Journal of Molecular Sciences
Article Title: Effect of Inflammatory Mediators Lipopolysaccharide and Lipoteichoic Acid on Iron Metabolism of Differentiated SH-SY5Y Cells Alters in the Presence of BV-2 Microglia
doi: 10.3390/ijms20010017
Figure Lengend Snippet: Determination of the concentration of secreted hepcidin from cell culture medium. The mature hepcidin content of each sample was determined with Hepcidin 25 bioactive ELISA Kit. ( A ) Hepcidin concentrations in the cell culture media of the LPS-treated SH-SY5Y monocultures and co-cultures. ( B ) Hepcidin productions of the LTA treated mono- and co-cultured SH-SY5Y cells measured from the culture medium. The columns represent mean values and error bars represent standard errors of the mean (SEM) of four independent determinations. The hepcidin contents of the control media did not change during the experiments therefore only one control was represented in the figures. Asterisk marks p < 0.01 between the mono- and co-cultures. Cross means p < 0.01 compared to the controls. ND means not detected.
Article Snippet: The mature hepcidin content of each sample was determined with
Techniques: Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay